| 出品公司: | Agilent |
|---|---|
| 载体名称: | pGL4.81[hRlucCP/Neo] |
| 载体又名: |
pGL4.81[hRlucCP/Neo]载体，pGL4.81[hRlucCP/Neo]质粒，pGL4.81[hRlucCP/Neo]载体质粒 pGL4.81载体，pGL4.81质粒，pGL4.81载体质粒， |
| 英文名称: |
pGL4.81[hRlucCP/Neo], pGL4.81[hRlucCP/Neo] Vector, pGL4.81[hRlucCP/Neo] Plasmid pGL4.81 pGL4.81 Vector, pGL4.81 Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 5.1 kb |
| 5’测序引物: | RVprimer3: CTAGCAAAATAGGCTGTCCC |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | G418新霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | pGL4.81[hRlucCP/Neo]载体说明书 |
| Genbank: | DQ188845 |
| 产品目录号: | 9PIE699 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.81[hRlucCP/Neo]载体质粒基本信息
pGL4.81[hRlucCP/Neo]质粒图谱载体图谱和pGL4.81[hRlucCP/Neo]载体序列质粒序列多克隆位点信息
![pGL4.81[hRlucCP/Neo]载体图谱](/uploads/载体图谱/pGL4.81载体图谱.png)
![pGL4.81[hRlucCP/Neo]多克隆位点](/uploads/载体图谱/pGL4.81[hRlucCP/Neo]多克隆位点.gif "pGL4.81[hRlucCP/Neo]载体多克隆位点")
pGL4.81[hRlucCP/Neo]质粒载体简介
pGL4.81[hRlucCP/Neo]载体（a–d）编码荧光素酶报告基因hRlucCP（海肾荧光素酶）。该载体旨在实现高表达并减少异常转录。此外，该载体还含有哺乳动物细胞选择性标记新霉素抗性标记，其中转录因子结合位点的数量已被减少，且该标记适用于哺乳动物密码子使用已优化。pGL4载体经过工程设计，其共有调控序列少于pGL3载体以及一个经过密码子优化以适应哺乳动物表达的合成报告基因。
pGL4.81[hRlucCP/Neo]载体是一个没有启动子的基础载体。然而，它包含一个多克隆位点允许克隆所选的启动子。hRlucCP报告基因包含两个蛋白质去稳定序列，即hCL1和hPEST。hRlucCP编码的蛋白质对变化的响应速度更快，幅度更大其转录活性不如hRluc基因，后者更为稳定。
pGL4.81[hRlucCP/Neo]质粒序列载体序列
pGL4.81[hRlucCP/Neo] snapgene格式序列:pGL4.81[hRlucCP/Neo].DNA
LOCUS pGL4.81[hRlucCP/Neo] 5061 bp DNA circular SYN 16-NOV-2005
DEFINITION Synthetic Renilla luciferase reporter vector pGL4.81[hRlucCP/Neo],
complete sequence.
ACCESSION DQ188845
VERSION DQ188845.1
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.81[hRlucCP/Neo]
ORGANISM Renilla luciferase reporter vector pGL4.81[hRlucCP/Neo]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5061)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5061)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2005) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..5061
/organism="Renilla luciferase reporter vector
pGL4.81[hRlucCP/Neo]"
/mol_type="other DNA"
/db_xref="taxon:346092"
misc_feature 1..70
/note="multiple cloning region"
CDS 100..1212
/note="synthetic Renilla luciferase; contains hPEST and
hCL1 destabilization sequences"
/codon_start=1
/product="hRlucCP"
/protein_id="ABA41677.1"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
regulatory 1249..1470
/regulatory_class="polyA_signal_sequence"
/note="SV40 late poly(A) signal"
regulatory 1518..1936
/regulatory_class="promoter"
/note="SV40 early enhancer/promoter"
CDS 1961..2755
/note="neomycin resistance"
/codon_start=1
/product="neomycin phosphotransferase"
/protein_id="ABA41678.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 2780..2828
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal"
primer_bind 2895..2914
/note="Reporter Vector primer 4 (RVprimer4) binding
region"
rep_origin 3152
/note="ColEI-derived plasmid replication origin"
gene complement(3943..4803)
/gene="bla"
CDS complement(3943..4803)
/gene="bla"
/note="ampicillin resistance"
/codon_start=1
/product="beta-lactamase"
/protein_id="ABA41679.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4908..5061
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal/transcriptional pause
site"
primer_bind 5010..5029
/note="Reporter Vector primer 3 (RVprimer3) binding
region"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgatcgaac aagacggcct
1981 ccatgctggc agtcccgcag cttgggtcga acgcttgttc gggtacgact gggcccagca
2041 gaccatcgga tgtagcgatg cggccgtgtt ccgtctaagc gctcaaggcc ggcccgtgct
2101 gttcgtgaag accgacctga gcggcgccct gaacgagctt caagacgagg ctgcccgcct
2161 gagctggctg gccaccaccg gcgtaccctg cgccgctgtg ttggatgttg tgaccgaagc
2221 cggccgggac tggctgctgc tgggcgaggt ccctggccag gatctgctga gcagccacct
2281 tgcccccgct gagaaggttt ctatcatggc cgatgcaatg cggcgcctgc acaccctgga
2341 ccccgctacc tgccccttcg accaccaggc taagcatcgg atcgagcgtg ctcggacccg
2401 catggaggcc ggcctggtgg accaggacga cctggacgag gagcatcagg gcctggcccc
2461 cgctgaactg ttcgcccgac tgaaagcccg catgccggac ggtgaggacc tggttgtcac
2521 acacggagat gcctgcctcc ctaacatcat ggtcgagaat ggccgcttct ccggcttcat
2581 cgactgcggt cgcctaggag ttgccgaccg ctaccaggac atcgccctgg ccacccgcga
2641 catcgctgag gagcttggcg gcgagtgggc cgaccgcttc ttagtcttgt acggcatcgc
2701 agctcccgac agccagcgca tcgccttcta ccgcttgctc gacgagttct tttaatgatc
2761 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
2821 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
2881 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
2941 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
3001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
3061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
3121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
3181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
3241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
3301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
3361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
3421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
3481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
3601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3901 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
3961 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
4021 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
4081 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
4141 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
4201 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
4261 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
4321 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
4381 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
4441 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
4501 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
4561 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
4621 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
4681 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
4741 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
4801 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
4861 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
4921 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
4981 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
5041 caggtgccag aacatttctc t
//
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